Comparative evaluation of microbial and chemical methods for assessing 4-chlorophenol biodegradation in soil

Authors

  • Zsuzsanna Magdolna Nagy
    Affiliation
    Budapest University of Technology and Economics, Department of Applied Biotechnology and Food Science
  • Katalin Gruiz
    Affiliation
    Budapest University of Technology and Economics, Department of Applied Biotechnology and Food Science
  • Mónika Molnár
    Affiliation
    Budapest University of Technology and Economics, Department of Applied Biotechnology and Food Science
  • Éva Fenyvesi
https://doi.org/10.3311/PPch.2167

Abstract

Reliable methods for assessing soil microflora and it’s activity are a prerequisite for successful technology planning and sustainable bioremediation of contaminated sites. The main objectives were to evaluate several microbiological soil-testing methods for characterizing the 4-chlorophenol biodegradation in the soil microcosm and to find the most appropriate methodology for testing biodegradation potential. The activity of the soil microflora were characterized by contaminant degrading cell concentration, dehydrogenase enzyme activity, three types of soil respiration and substrate utilization of the microbial community. The contaminant concentrations were measured by exhaustive extraction and by non-exhaustive cyclodextrin extraction. Most of the applied biological methods were found to be reliable indicators of chlorophenol biodegradation in soil, and can be useful as a pre-implementation methodology to support technology selection and design. The microbial community analyses by BIOLOG EcoPlateTM provided very good results and can be suitable for use in biodegradation assessment and evaluation in soil.

Keywords:

4-chlorophenol, integrated methodology, soil microbial community, BIOLOG EcoPlate™, non-exhaustive cyclodextrin extraction

Citation data from Crossref and Scopus

Published Online

2013-06-26

How to Cite

Nagy, Z. M., Gruiz, K., Molnár, M., Fenyvesi, Éva “Comparative evaluation of microbial and chemical methods for assessing 4-chlorophenol biodegradation in soil”, Periodica Polytechnica Chemical Engineering, 57(1-2), pp. 25–35, 2013. https://doi.org/10.3311/PPch.2167

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Articles