Application of Gas Chromatography – Flame Ionization Detection to Study Cellular Incorporation of Dietary Trans Fatty Acids of Medical Importance

Authors

  • Anna Somogyi
    Affiliation
    Department of Molecular Biology, Institute of Biochemistry and Molecular Biology, Faculty of Medicine, Semmelweis University, H-1428 Budapest, P.O.B. 2, Hungary
  • Judit Mátyási
    Affiliation
    Department of Inorganic and Analytical Chemistry, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, H-1521 Budapest, P.O.B. 91, Hungary
  • Zsófia Gór-Nagy
    Affiliation
    Department of Inorganic and Analytical Chemistry, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, H-1521 Budapest, P.O.B. 91, Hungary
  • Farkas Sarnyai
    Affiliation
    Department of Molecular Biology, Institute of Biochemistry and Molecular Biology, Faculty of Medicine, Semmelweis University, H-1428 Budapest, P.O.B. 2, Hungary
  • Miklós Csala
    Affiliation
    Department of Molecular Biology, Institute of Biochemistry and Molecular Biology, Faculty of Medicine, Semmelweis University, H-1428 Budapest, P.O.B. 2, Hungary
  • Blanka Tóth
    Affiliation
    Department of Inorganic and Analytical Chemistry, Faculty of Chemical Technology and Biotechnology,Budapest University of Technology and Economics, H-1521 Budapest, P.O.B. 91, Hungary
https://doi.org/10.3311/PPch.16646

Abstract

Putative health effects of dietary trans fatty acids (TFAs) receive a growing attention; while very little is known about the metabolism of these special food components. In vitro studies carried out in cultured cells provide an efficient and standardizable approach to follow the metabolic fate of TFAs, but it requires suitable techniques for the quantitative measurement of FAs in cell samples. Here, the development and validation of a simple and reliable method for the quantification of a group of relevant FAs by gas chromatography and flame ionization detection is presented. Sample preparation used a fast one-step and chloroform-free process for simultaneous extraction and esterification, and chromatographic separation was achieved in 25 min using a Zebron ZB-88 capillary column. A linear calibration (of R2 >0.99) was obtained in the concentration range of 1-200 µg/mL for each FA. Recovery rate was 82 % for samples of non-esterified FAs and >95 % for complex lipids, such as ceramides, diglycerides and triglycerides. The LOD and LOQ were below 0.5 µg/mL, and a robust method precision was achieved (RSD % was below 6 % for each lipid classes). The present method was also tested on a cultured cell line with or without FA treatment at close to physiological concentration, and the observed changes in the metabolite concentration levels revealed characteristic differences between the metabolism of cis and trans unsaturated FAs.

Keywords:

cell culture, fast method, method validation, trans fatty acid

Citation data from Crossref and Scopus

Published Online

2021-02-02

How to Cite

Somogyi, A., Mátyási, J., Gór-Nagy, Z., Sarnyai, F., Csala, M., Tóth, B. “Application of Gas Chromatography – Flame Ionization Detection to Study Cellular Incorporation of Dietary Trans Fatty Acids of Medical Importance”, Periodica Polytechnica Chemical Engineering, 65(2), pp. 149–157, 2021. https://doi.org/10.3311/PPch.16646

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